Biocompatible chitosan/polyethylene glycol/multi-walled carbon nanotube composite scaffolds for neural tissue engineering / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Carbon nanotube (CNT) composite materials are very attractive for use in neural tissue engineering and biosensor coatings. CNT scaffolds are excellent mimics of extracellular matrix due to their hydrophilicity, viscosity, and biocompatibility. CNTs can also impart conductivity to other insulating materials, improve mechanical stability, guide neuronal cell behavior, and trigger axon regeneration. The performance of chitosan (CS)/polyethylene glycol (PEG) composite scaffolds could be optimized by introducing multi-walled CNTs (MWCNTs). CS/PEG/CNT composite scaffolds with CNT content of 1%, 3%, and 5% (1%=0.01 g/mL) were prepared by freeze-drying. Their physical and chemical properties and biocompatibility were evaluated. Scanning electron microscopy (SEM) showed that the composite scaffolds had a highly connected porous structure. Transmission electron microscope (TEM) and Raman spectroscopy proved that the CNTs were well dispersed in the CS/PEG matrix and combined with the CS/PEG nanofiber bundles. MWCNTs enhanced the elastic modulus of the scaffold. The porosity of the scaffolds ranged from 83% to 96%. They reached a stable water swelling state within 24 h, and swelling decreased with increasing MWCNT concentration. The electrical conductivity and cell adhesion rate of the scaffolds increased with increasing MWCNT content. Immunofluorescence showed that rat pheochromocytoma (PC12) cells grown in the scaffolds had characteristics similar to nerve cells. We measured changes in the expression of nerve cell markers by quantitative real-time polymerase chain reaction (qRT-PCR), and found that PC12 cells cultured in the scaffolds expressed growth-associated protein 43 (GAP43), nerve growth factor receptor (NGFR), and class III β‍-tubulin (TUBB3) proteins. Preliminary research showed that the prepared CS/PEG/CNT scaffold has good biocompatibility and can be further applied to neural tissue engineering research.

A review of intercomparison of measurement results by gamma-ray spectrometry in three laboratories in China and Japan / 中华放射医学与防护杂志

Objective:To improve the radionuclide measurement technology by γ-spectrometry throuth a review of the intercomparisons between China and Japan results over the past 20 years.Methods:Before the beginning of each intercomparison, one of participating laboratories was agreed to be responsible for preparing the samples for the intercomparison. By intercomparison of the measurement results arnong the laboratories some potential problems during the sample pre-trement or analysis were found and solved.Results:The intercomparisons had been carried out for 11 times over the past 20 years. Totally 290 measured and analyzed results had been obtained from 20 samples of six categories. The proportion 95.5% of measurement results were in agreement with each other within the deviation range.Conclusions:The measuring and analytical capability of the laboratories involved were tested. The intercomparisons also provide long-term quality assurance measures for the radionuclide measurement and analysis in each laboratory.

Chinese neonatal birth weight curve for different gestational age / 中华儿科杂志

<p><b>OBJECTIVE</b>Since 1986, the reference of birth weight for gestational age has not been updated. The aim of this study was to set up Chinese neonatal network to investigate the current situation of birth weight in China, especially preterm birth weight, to develop the new reference for birth weight for gestational age and birth weight curve.</p><p><b>METHOD</b>A nationwide neonatology network was established in China. This survey was carried out in 63 hospitals of 23 provinces, municipalities and autonomous regions. We continuously collected the information of live births in participating hospitals during the study period of 2011-2014. Data describing birth weight and gestational age were collected prospectively. Newborn's birth weight was measured by electronic scale within 2 hours after birth when baby was undressed. The evaluation of gestational age was based on the combination of mother's last menstrual period, ultrasound in first trimester and gestational age estimation by gestational age scoring system.</p><p><b>STATISTICAL ANALYSIS</b>the growth curve was drawn by using LMSP method, which was conducted in GAMLSS 1.9-4 software package in R software 2.11.1.</p><p><b>RESULT</b>A total of 159 334 newborn infants were enrolled in this study. There were 84 447 male and 74 907 female. The mean birth weight was (3 232 ± 555) g, the mean birth weight of male newborn was (3 271 ± 576) g, the mean weight of female newborn was (3 188 ± 528) g. The test of the variables' distribution suggested that the distribution of gestational age and birth weight did not fit the normal distribution, the optimal distribution for them was BCT distribution. The Q-Q plot test and worm plot test suggested that this curve fitted the distribution optimally. The male and female neonatal birth weight curve was developed using the same method.</p><p><b>CONCLUSION</b>Using GAMLSS method to establish nationwide neonatal birth weight curve, and the first time to update the birth weight reference in recent 28 years.</p>

Correlation of RBP4 and omentin-1with ischemic stroke and carotid artery stenosis / 天津医药

Objective To investigate the correlation of serum levels of retinol binding protein 4 (RBP4) and andomen?tin-1 with ischemic stroke and carotid artery stenosis. Methods Healthy adult (n=50) were selected as the control group while ischemic stroke patients with different degrees of carotid artery stenosis (n=143) were enrolled as the disease group. According to the ultrasonic Doppler performance, these ischemic stroke patients were divided into three groups:mild steno?sis group (carotid artery stenosis was normal or less than 50%, n=67), moderate stenosis group (carotid artery stenosis was 50%to 70%, n=45), severe stenosis group (carotid artery stenosis was 70%to total occlusion, n=31). BMI index, hyperten?sion, total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL) and fasting blood glucose (FPG) were detected by Automatic biochemical analyzer and serum levels of RBP4 and omentin-1 were measured by ELISA. Results Hypertensive population in carotid artery stenosis group was larger than that in control group (P<0.05). Serum TC, TG, LDL, FPG and RBP4 levels were significantly higher while omentin-1 level decreased remarkably in disease group than those in the control group (P<0.05). Logistic regression analysis showed that TC and RBP4 were the risk factors of ischemic stroke with carotid artery stenosis, while omentin-1 was a protective factor. Conclusion RBP4 is a risk factor for ischemic stroke with carotid artery stenosis and omentin-1 is a protective factor.

Strengthen the postgraduate programs to improve scientific research / 中华医学科研管理杂志

Postgraduate students make an important contribution to scientific research work,and they are also important for discipline development,scientific research subject verification and talents cultivation.Besides,the problems existing in postgraduate training are analysed,which further expounded the role postgraduate training in the overall research potential of the institute.

Clinical study of epileptic aura / 中华神经科杂志

Objective To study the prevalence and clinical features of auras in epilepsy patients,and to evaluate the diagnosis criteria of epileptic auras.Methods All 1028 patients with epilepsy who were treated in the First Hospital of PekiIIg University from January 2007 to January 2008 were enrolled in this retrospective study.Seizure types were diagnosed in accordance with the seizure classification scheme proposed by the International League Against Epilepsy(1981).We studied the incidence rate,clinical features and presentations of electroencephalogram(EEG)and neuroimaging of epileptic auras.Student ttest was used to assess the statistic difference of the onset age of partial seizure in patients with and without auras.The chi-squaretestWas usedto statistically analyze the EEGand neuroimaging results in patients with partial seizure and the occurrence percentages of abdominal aura in lateral temporal lobe epilepsy(LTLE)and mesial temporal lobe epilepsy(MTLE).Results Four hundred and eighty-four patients(66.8 % of 725 patients)with partial seizures reported epileptic auras.None of the 303 patients with generalized seizures reported auras.Sixty-four patients experienced two or more types of auras.Fourteen patients had aura status. Of all enrolled patients,53.2 % and 21.1 % showed abnormal EEG and neuroimaging,respectively.Of 484 partial epileptic patients with auras,59.1 % and 26.0 % presented abnormal EEG and neuroimagings,respectively.The percentage of abdomen aura in patients with MTLE was significantly higher than those with LTLE(x2=170.877,P<0.01).Condusion Analysis of auras may help in classifying and localizing epilepsy and also in planning treatment.

Relationship between iron overloading and heme oxygenase-1 after rat intracerebral hemorrhage / 中华老年医学杂志

Objective To investigate whether iron mass induces HO-1 overexpression and explore the role of HO-1 in rat intracerebral hemorrhage(ICH). Methods In this study,144 hydrated chloride aldehyde-anesthetized Sprague- Dawley rats were used,autologous blood were injected into the right caudate nucleus to establish the ICH model.Saline injection and health were served as controls.Deferoxamine(DFO)with an intraperitoneal injection served as intervention group.Enhanced Perl's reaction was used for iron staining and brain iron deposits were determined.Brain HO-1 level were examined by immunohistochemical analysis and reverse transcription polymerase chain reaction(RT-PCR). Results There was a 21-fold increase in iron deposits around the hematoma 7 days after the infusion of 100 μl of autologous blood.Markedly increased levels of perihematomal HO-1 immunoreactivity and HO-1 mRNA in all ICH rats were detected at 3-14 days.The addition of DFO significantly reduced iron deposits in the ipsilateral basal ganglia at 7-14 days after ICH.DFO also inhibited HO-1 overexpression at day 7,14.Correlations test showed that there were positive correlations of iron sediments with HO-1mRNA(r=0.647)and HO-1 immunopositive cells(r=0.209). Conclusions ICH causes iron accumulation in the brain.Iron overloading may induce HO-1 upregulation after ICH.Ratherly,the HO-1 moderate increasing possibly fits with the events,whereas HO-1 overexpression may result in its dysfunction.It may be prudent to intervene ICH with HO-1 inhibitor.

Chinicd Effect of acute Cerebral Infarction Treated with FDP / 医学研究杂志

Objective To observe the effect of acute cerebral infarction treated with FPD.Methods 50 cases of acute cerebral infarction patient were treated with FPD as observation group according to the diagnostic standard that made in 1986,Other 50 cases of comparable patirnt were as control so that to compare the curative effect.Results The nerve function deficiency in observation group was maredry hower than.Concusions FDP could reach an obvions effect that treat acute cercbral infarction via some aspecta.

Improvement of high glucose toxicity-induced insulin desensitivity by nitric oxide in diabetic rats / 中华内分泌代谢杂志

Objective To observe whether nitric oxide (NO) improves high glucose toxicity induced insulin resistance in streptozotocin (STZ) induced diabetic rats. Methods The changes of metabolic clearance rate for glucose (MCR), as the index of insulin effect, were observed in 24 STZ induced diabetic rats and 24 normal rats in awake condition treated by sodium nitroprusside (SNP), N G monomethyl L arginine (LNMMA), adenosine or saline duringeuglycemichyperinsulinemiaclampprocedure.Results MCR in saline treated diabetic rats 〔(7.2?0.8)ml?kg -1 ?min -1 〕 wassignificantlylowerthanthat in normol control rats 〔(18.0?1.8)ml?kg -1 ?min -1 , P